Dynamic Light Scattering Application to Study Protein Interactions in Electrolyte Solutions
نویسندگان
چکیده
منابع مشابه
Dynamic light scattering application to study protein interactions in electrolyte solutions.
The concentration dependence of the diffusion coefficient of particles suspended in solution depends primarily on the occupied volume fraction and on repulsive and attractive forces. This dependency is expressed by the interaction parameter, which can be assessed experimentally by light scattering measurements and have been determined for the diffusion coefficient of BSA under different salt co...
متن کاملDynamic light scattering and application to proteins in solutions
Dynamic light scattering (DLS) measures time-dependent fluctuations in the scattering intensity arising from particles undergoing random Brownian motion. Diffusion coefficient and particle size information can be obtained from the analysis of these fluctuations. More specifically, the method provides the ability to measure size characteristics of proteins in a liquid medium. Proteins consist of...
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Many drinkable dairy products added with green tea polyphenols have been launched by food industries because epidemiological studies suggest an association between the prevention of diseases and the consumption of polyphenol-rich beverages or foods. However, polyphenols have a significant affinity for proteins that leads to formation of soluble complexes, which can grow in size and even form se...
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Protein interactions in undersaturated and supersaturated solutions were investigated using static and dynamic light scattering and small angle x-ray scattering. A morphodrom of lysozyme crystals determined at 35 degrees C and pH = 4.6 was used as a guideline in selecting the protein and precipitant concentrations. The osmotic second virial coefficient, B(22), was determined by static and dynam...
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We report the dynamic light scattering study of the gelation of aqueous solutions of the biopolymer, pectin, induced by the addition of calcium chloride. The time correlation function data are analyzed under the framework of the coupling model. As the solution enters the semidilute regime where gelation sets in, the relaxation process shows a stretched exponential behavior. The stretching expon...
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ژورنال
عنوان ژورنال: Journal of Biological Physics
سال: 2004
ISSN: 0092-0606,1573-0689
DOI: 10.1007/s10867-004-0997-z